Trinity Biotech

Trinity Biotech provides a range of tests and components for the quantitative and qualitative determination of Glucose-6-Phosphate Dehydrogenase (G-6-PDH) Deficiency:

• Quantitative Kinetic tests
• Qualitative screening test

Glucose-6-Phosphate Dehydrogenase (G-6-PDH) is an X-linked disorder is the most common type of haemolytic anaemia due to an intrinsic red cell enzyme defect. Males who inherit an abnormal gene are invariably affected. Heterozygote females usually have approximately 50% G6PD enzyme activity; the random Lyonisation of X chromosomes means that rarely carrier females may be severely affected. It is most common in the Mediterranean, the Middle East, South East Asia and West Africa. It is rare among Caucasians.

G-6-PDH Deficiency Screen by Spot Test

METHOD
Spot Test, Visual Fluorescence (qualitative)

PRINCIPLE
The reaction mixture containing glucose-6-phosphate+NADP (Not Fluorescent) and blood is incubated and, at timed intervals, drops of the mixture are applied to filter paper. The spots are visually inspected under long-wavelength (320-420nm) ultraviolet light. The observed rate of appearance of bright Fluorescence is proportional to the blood G-6-PDH activity.

Product information

DIAGNOSTIC IMPLICATIONS

Presentation is usually with an acute episode of intravascular haemolysis on exposure to certain drugs, infection or acute illness. Mediterranean forms may present with neonatal jaundice. The condition is also known as favism as sudden haemolysis may be precipitated by the ingestion of fava beans – Vicia faba. The deficiency is generally less severe in Africans and favism is rare in this population.